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ps6(s240/244) (cell signaling, cat# 5364  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc ps6(s240/244) (cell signaling, cat# 5364
    Ps6(s240/244) (Cell Signaling, Cat# 5364, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ps6+s240+244/bio_rxiv__64898__2026__04__04__716461-206-119-120
    Average 86 stars, based on 1 article reviews
    ps6(s240/244) (cell signaling, cat# 5364 - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Western Blot:

    Article Title: Neuron-specific isoform of PGC-1α regulates neuronal metabolism and brain aging
    Article Snippet: .. Antibodies used for western blotting were: pGSK3b(S9) (#9336; Cell Signaling Technologies), GSK3b (9315S; Cell Signaling Technologies), pCREB (S133) (ab32096; Abcam), CREB (#9104; Cell Signaling Technologies), pAMPK (T172) (#2535S; Cell Signaling Technologies), AMPK (#2532S; Cell Signaling Technologies), pIRS-1 (S636) (#2388S; Cell Signaling Technologies), IRS-1 (#2382S; Cell Signaling Technologies), pAKT (T308) (#13038S; Cell Signaling Technologies), AKT (#4691; Cell Signaling Technologies), pS6 (S240/244) (#2215S; Cell Signaling Technologies), S6 (#2217S; Cell Signaling Technologies), pERK1/2 (T202/ Y204) (#4370S; Cell Signaling Technologies), ERK1/2 (#4695S; Cell Signaling Technologies). rimary antibodies used for immunofluorescence were anti-a-Tubulin primary antibody (1:200) (Sigma-Aldrich #T6199) and Tomm20 (1:84.2) (Abcam ab56783). ..

    Immunofluorescence:

    Article Title: Neuron-specific isoform of PGC-1α regulates neuronal metabolism and brain aging
    Article Snippet: .. Antibodies used for western blotting were: pGSK3b(S9) (#9336; Cell Signaling Technologies), GSK3b (9315S; Cell Signaling Technologies), pCREB (S133) (ab32096; Abcam), CREB (#9104; Cell Signaling Technologies), pAMPK (T172) (#2535S; Cell Signaling Technologies), AMPK (#2532S; Cell Signaling Technologies), pIRS-1 (S636) (#2388S; Cell Signaling Technologies), IRS-1 (#2382S; Cell Signaling Technologies), pAKT (T308) (#13038S; Cell Signaling Technologies), AKT (#4691; Cell Signaling Technologies), pS6 (S240/244) (#2215S; Cell Signaling Technologies), S6 (#2217S; Cell Signaling Technologies), pERK1/2 (T202/ Y204) (#4370S; Cell Signaling Technologies), ERK1/2 (#4695S; Cell Signaling Technologies). rimary antibodies used for immunofluorescence were anti-a-Tubulin primary antibody (1:200) (Sigma-Aldrich #T6199) and Tomm20 (1:84.2) (Abcam ab56783). ..



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    ps6(s240/244) (cell signaling, cat# 5364  (Cell Signaling Technology Inc)
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    ( A ) Levels of phosphorylated SYK induced upon BCR stimulation with α-IgG, gp34 1-179 and gp34 1-179W65F over the indicated time points, was examined by flow cytometry and depicted as MFI of three biological replicates. A two-way ANOVA (with mixed-effects analysis) was performed for statistical analysis, * P = 0.0416, ** P = 0.0388. Data are shown as the mean ± standard of the mean. ( B ) B cells were stimulated with α-IgG, gp34 1-179 , and gp34 1-179W65F for 60 min at 37 °C. The cells were lysed and separated on a 12% SDS–PAGE. An α-DOK3 antibody was used to detect DOK3 expressed by B cells in each condition. Results are representative of two independent experiments. β-actin served as loading control. ( C ) Gating strategy showing IgD + B cells expressing phosphorylated S6. ( D ) The histograms show the percentages of <t>pS6</t> + cells in IgD + B cells upon stimulation with gp34 1-179 , gp34 1-179W65F or α-BCR. Bar graphs depict frequency of pS6 + cells in IgD + B cells in three biological replicates. A non-parametric Kruskal–Wallis test was performed to analyze the differences in each group (** P = 0.0012). Data are shown as the mean ± standard of the mean.
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    ( A ) Levels of phosphorylated SYK induced upon BCR stimulation with α-IgG, gp34 1-179 and gp34 1-179W65F over the indicated time points, was examined by flow cytometry and depicted as MFI of three biological replicates. A two-way ANOVA (with mixed-effects analysis) was performed for statistical analysis, * P = 0.0416, ** P = 0.0388. Data are shown as the mean ± standard of the mean. ( B ) B cells were stimulated with α-IgG, gp34 1-179 , and gp34 1-179W65F for 60 min at 37 °C. The cells were lysed and separated on a 12% SDS–PAGE. An α-DOK3 antibody was used to detect DOK3 expressed by B cells in each condition. Results are representative of two independent experiments. β-actin served as loading control. ( C ) Gating strategy showing IgD + B cells expressing phosphorylated S6. ( D ) The histograms show the percentages of <t>pS6</t> + cells in IgD + B cells upon stimulation with gp34 1-179 , gp34 1-179W65F or α-BCR. Bar graphs depict frequency of pS6 + cells in IgD + B cells in three biological replicates. A non-parametric Kruskal–Wallis test was performed to analyze the differences in each group (** P = 0.0012). Data are shown as the mean ± standard of the mean.
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    ( A ) Levels of phosphorylated SYK induced upon BCR stimulation with α-IgG, gp34 1-179 and gp34 1-179W65F over the indicated time points, was examined by flow cytometry and depicted as MFI of three biological replicates. A two-way ANOVA (with mixed-effects analysis) was performed for statistical analysis, * P = 0.0416, ** P = 0.0388. Data are shown as the mean ± standard of the mean. ( B ) B cells were stimulated with α-IgG, gp34 1-179 , and gp34 1-179W65F for 60 min at 37 °C. The cells were lysed and separated on a 12% SDS–PAGE. An α-DOK3 antibody was used to detect DOK3 expressed by B cells in each condition. Results are representative of two independent experiments. β-actin served as loading control. ( C ) Gating strategy showing IgD + B cells expressing phosphorylated S6. ( D ) The histograms show the percentages of <t>pS6</t> + cells in IgD + B cells upon stimulation with gp34 1-179 , gp34 1-179W65F or α-BCR. Bar graphs depict frequency of pS6 + cells in IgD + B cells in three biological replicates. A non-parametric Kruskal–Wallis test was performed to analyze the differences in each group (** P = 0.0012). Data are shown as the mean ± standard of the mean.
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    ( A ) Levels of phosphorylated SYK induced upon BCR stimulation with α-IgG, gp34 1-179 and gp34 1-179W65F over the indicated time points, was examined by flow cytometry and depicted as MFI of three biological replicates. A two-way ANOVA (with mixed-effects analysis) was performed for statistical analysis, * P = 0.0416, ** P = 0.0388. Data are shown as the mean ± standard of the mean. ( B ) B cells were stimulated with α-IgG, gp34 1-179 , and gp34 1-179W65F for 60 min at 37 °C. The cells were lysed and separated on a 12% SDS–PAGE. An α-DOK3 antibody was used to detect DOK3 expressed by B cells in each condition. Results are representative of two independent experiments. β-actin served as loading control. ( C ) Gating strategy showing IgD + B cells expressing phosphorylated S6. ( D ) The histograms show the percentages of <t>pS6</t> + cells in IgD + B cells upon stimulation with gp34 1-179 , gp34 1-179W65F or α-BCR. Bar graphs depict frequency of pS6 + cells in IgD + B cells in three biological replicates. A non-parametric Kruskal–Wallis test was performed to analyze the differences in each group (** P = 0.0012). Data are shown as the mean ± standard of the mean.
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    ( A ) Levels of phosphorylated SYK induced upon BCR stimulation with α-IgG, gp34 1-179 and gp34 1-179W65F over the indicated time points, was examined by flow cytometry and depicted as MFI of three biological replicates. A two-way ANOVA (with mixed-effects analysis) was performed for statistical analysis, * P = 0.0416, ** P = 0.0388. Data are shown as the mean ± standard of the mean. ( B ) B cells were stimulated with α-IgG, gp34 1-179 , and gp34 1-179W65F for 60 min at 37 °C. The cells were lysed and separated on a 12% SDS–PAGE. An α-DOK3 antibody was used to detect DOK3 expressed by B cells in each condition. Results are representative of two independent experiments. β-actin served as loading control. ( C ) Gating strategy showing IgD + B cells expressing phosphorylated S6. ( D ) The histograms show the percentages of pS6 + cells in IgD + B cells upon stimulation with gp34 1-179 , gp34 1-179W65F or α-BCR. Bar graphs depict frequency of pS6 + cells in IgD + B cells in three biological replicates. A non-parametric Kruskal–Wallis test was performed to analyze the differences in each group (** P = 0.0012). Data are shown as the mean ± standard of the mean.

    Journal: EMBO Molecular Medicine

    Article Title: A viral glycoprotein targets IgG + memory B cells to mediate humoral immune evasion

    doi: 10.1038/s44321-026-00372-1

    Figure Lengend Snippet: ( A ) Levels of phosphorylated SYK induced upon BCR stimulation with α-IgG, gp34 1-179 and gp34 1-179W65F over the indicated time points, was examined by flow cytometry and depicted as MFI of three biological replicates. A two-way ANOVA (with mixed-effects analysis) was performed for statistical analysis, * P = 0.0416, ** P = 0.0388. Data are shown as the mean ± standard of the mean. ( B ) B cells were stimulated with α-IgG, gp34 1-179 , and gp34 1-179W65F for 60 min at 37 °C. The cells were lysed and separated on a 12% SDS–PAGE. An α-DOK3 antibody was used to detect DOK3 expressed by B cells in each condition. Results are representative of two independent experiments. β-actin served as loading control. ( C ) Gating strategy showing IgD + B cells expressing phosphorylated S6. ( D ) The histograms show the percentages of pS6 + cells in IgD + B cells upon stimulation with gp34 1-179 , gp34 1-179W65F or α-BCR. Bar graphs depict frequency of pS6 + cells in IgD + B cells in three biological replicates. A non-parametric Kruskal–Wallis test was performed to analyze the differences in each group (** P = 0.0012). Data are shown as the mean ± standard of the mean.

    Article Snippet: Phosphorylated proteins were detected using the following antibodies: pLyn (Tyr 397), pPDK1 (S241) (clone C49H2), pAKT (S473), pAKT (T308), pS6 (S240/244) (all Cell Signaling Technology), pPLCγ2 (pY759) (Miltenyi Biotec), and pSyk (Y348) (BD Biosciences).

    Techniques: Flow Cytometry, SDS Page, Control, Expressing

    ( A ) Phosphorylation of PDK1 and AKT at the indicated amino acid residues after stimulation of isolated B cells with 10 µg/mL of α-BCR, gp34 1-179 , or gp34 1-179W65F for 10 min. Bars represent percentages of PDK1-positive IgG + B cells or MFI of pAKT (T308) and pAKT (S473) in IgG + B cells. At least three replicates were analyzed, and differences were analyzed using one-way ANOVA for PDK1 (**** P < 0.0001) or a Kruskal–Wallis test for pAKT (T308, * P = 0.0343) and pAKT (S473, * P = 0.023). Error bars + standard deviation of the mean. ( B ) B cells were incubated for 15 min with 2 µM Wortmannin or DMSO prior to stimulation with α-BCR, gp34 1-179 , or gp34 1-179W65F for 10 min. pAKT (S473) was analyzed by flow cytometry ( n = 2). ( C ) Gating strategy to identify IgG + cells in the live B cells gate expressing phosphorylated S6. ( D ) Histograms show the MFI of p-S6 within the IgG + B cells gate. Bars represent the percentage of pS6-positive cells within the IgG + B cell population. Data represent five independent experiments; error bars indicate standard deviation, and differences between groups were analyzed using one-way ANOVA (** P = 0.0047).

    Journal: EMBO Molecular Medicine

    Article Title: A viral glycoprotein targets IgG + memory B cells to mediate humoral immune evasion

    doi: 10.1038/s44321-026-00372-1

    Figure Lengend Snippet: ( A ) Phosphorylation of PDK1 and AKT at the indicated amino acid residues after stimulation of isolated B cells with 10 µg/mL of α-BCR, gp34 1-179 , or gp34 1-179W65F for 10 min. Bars represent percentages of PDK1-positive IgG + B cells or MFI of pAKT (T308) and pAKT (S473) in IgG + B cells. At least three replicates were analyzed, and differences were analyzed using one-way ANOVA for PDK1 (**** P < 0.0001) or a Kruskal–Wallis test for pAKT (T308, * P = 0.0343) and pAKT (S473, * P = 0.023). Error bars + standard deviation of the mean. ( B ) B cells were incubated for 15 min with 2 µM Wortmannin or DMSO prior to stimulation with α-BCR, gp34 1-179 , or gp34 1-179W65F for 10 min. pAKT (S473) was analyzed by flow cytometry ( n = 2). ( C ) Gating strategy to identify IgG + cells in the live B cells gate expressing phosphorylated S6. ( D ) Histograms show the MFI of p-S6 within the IgG + B cells gate. Bars represent the percentage of pS6-positive cells within the IgG + B cell population. Data represent five independent experiments; error bars indicate standard deviation, and differences between groups were analyzed using one-way ANOVA (** P = 0.0047).

    Article Snippet: Phosphorylated proteins were detected using the following antibodies: pLyn (Tyr 397), pPDK1 (S241) (clone C49H2), pAKT (S473), pAKT (T308), pS6 (S240/244) (all Cell Signaling Technology), pPLCγ2 (pY759) (Miltenyi Biotec), and pSyk (Y348) (BD Biosciences).

    Techniques: Phospho-proteomics, Isolation, Standard Deviation, Incubation, Flow Cytometry, Expressing